SELECTED PUBLICATIONS

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Structure and Lateral Organization of Phosphatidylinositol 4,5-bisphosphate

L. Araújo and F. Fernandes, Molecules 2020

(Selected for Cover of Special Issue)

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SEDS–bPBP pairs direct lateral and septal peptidoglycan synthesis in Staphylococcus aureus

Reichmann N.T., Tavares, A.C., Saraiva B.M. Jousselin A., Reed P., Pereira A.R., Monteiro J.M., Sobral R.G., VanNieuwenhze M.S.,
Fernandes F., Pinho M.G.,
Nature Microbiology  4, 1368–1377 2019

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Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

Sousa T., Castro R.E., Coutinho A., Rodrigues, C.M.P., Prieto M., Fernandes F. In: Vinken M. (eds) Experimental Cholestasis Research. Methods in Molecular Biology vol 1981, 2019.

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Modulation of phase separation at the micron scale and nanoscale in giant polymer/lipid hybrid unilamellar vesicles (GHUVs)

Dao, T.P.T., Fernandes F., Ibarboure E., Ferji K, M. Prieto, Sandre O., Le Meins, J. F.  Soft Matter 13, 627. 2017

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Membrane Order Is a Key Regulator of Divalent Cation-Induced Clustering of PI(3,5)P2 and PI(4,5)P2

Sousa T., Castro R.E., Coutinho A., Rodrigues, C.M.P., Prieto M., Fernandes F.  Langmuir 33, 43, 12463. 2017

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Mixing Block Copolymers with Phospholipids at the Nanoscale: From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains

Dao, T.P.T., Brûlet, A., Fernandes, F., Er-Rafik M., Ferji K., Schweins R., Chapel J.-P.P., Fedorov A., Schmutz M., Prieto M. Sandre O., Le Meins J.-F.F. Langmuir 33, 1705. 2017

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Deoxycholic acid modulates cell death signaling through changes in mitochondrial membrane properties (Cover of November 2015 issue)

Sousa T., Castro R.E., Pinto S,, Coutinho A., Lucas S.D., Moreira R., Rodrigues, C.M.P., Prieto M., Fernandes F. Journal of Lipid Research 56, 2158. 2015

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Ca2+ induces PI(4,5)P2 clusters on lipid bilayers at physiological PI(4,5)P2 and Ca2 + concentrations

Sarmento M., Coutinho A., Fedorov A., Prieto M., Fernandes F. Biochimica et Biophysica Acta (BBA) - Biomembranes 1838: 822. 2014

OTHER PUBLICATIONS

 

R. Russo  R. Padanha  F. Fernandes  L. Veiros  F. Corzana  P.M.P. Gois. 2020. Engineering Boron Hot Spots for the Site‐Selective Installation of Iminoboronates on Peptide Chains. Chem. Eur. J. doi: 10.1002/chem.202002675. 

 

C.I.C. Crucho, J. Avo, R. Nobuyasu, S.N. Pinto, F. Fernandes, J.C. Lima, M.N. Berberan-Santos, F.B. Dias. 2020. Silica nanoparticles with thermally activated delayed fluorescence for live cell imaging. Materials Science and Engineering: C 109: 110528. doi: 10.1016/j.msec.2019.110528

 

L.M.S: Loura, M. Prieto, F. Fernandes. 2019. Forster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid Selectivity. In: Kleinschmidt J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 2003. Humana, New York, NY.

T.P.T. Dao, K. Ferji, F. Fernandes, M. Prieto, S. Lecommandoux, E. Ibarboure, O. Sandre, and J. F. Le Meins. 2019. Giant hybrid polymer/lipid vesicles. In: R. Dimova, C. Marques (eds) The Giant Vesicle Book. CRC Press - Taylor and Francis group, pp.543-561.

S.N. Pinto, S.A. Dias, A.F. Cruz, D. Mil-Homens, F. Fernandes, J. Valle, D. Andreu, M. Prieto, M.A.R.B. Castanho, A.Coutinho, A.S. Veiga. 2019. The mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms, Journal of Antimicrobial Chemotherapy 74: 2617-2625. doi: 10.1093/jac/dkz223

C.P. Godinho, C.S. Prata, S.N. Pinto, C. Cardoso, N.M. Bandarra, F. Fernandes, I. Sa-Correia. 2018. Pdr18 is involved in yeast response to acetic acid stress counteracting the decrease of plasma membrane ergosterol content and order, Sci. Rep. 8:7860. doi: 10.1038/s41598-018-26128-7

T.P. Dias, S.N. Pinto, J.I. Santos, T.G. Fernandes, F. Fernandes, Margarida Diogo, M. Prieto, J.M.S. Cabral. 2018. Biophysical study of human induced pluripotent stem cell-derived cardiomyocyte structural maturation during long-term culture. Biochem. Biophys. Res. Commun 499: 611-617. doi: 10.1016/j.bbrc.2018.03.198

N. Bernardes, A.R. Garizo, S.N.Pinto, B. Caniço, C. Perdigão, F. Fernandes, A.M. Fialho. 2018. Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs, Cell Cycle 17: 1649-1666. doi: 0.1080/15384101.2018.1489178

C.P. Godinho, C.S. Prata, S.N. Pinto, C. Cardoso, N.M. Bandarra, F. Fernandes, I. Sá-Correia. 2018. Pdr18 is involved in yeast response to acetic acid stress counteracting the decrease of plasma membrane ergosterol content and order, Sci. Rep. 8: 7860. doi:10.1038/s41598-018-26128-7.

 

N. Bernardes, A.R. Garizo, S.N.Pinto, B. Caniço, C. Perdigão, F. Fernandes, A.M. Fialho. 2018. Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs, Cell Cycle 17: 1649-1666. doi: 10.1080/15384101.2018.1489178.

M.A.M. Videira, S.A.L. Lobo, L.S.O. Silva, D.J. Palmer, M.J. Warren, M. Prieto, A. Coutinho, F.L. Sousa, F. Fernandes, L.M. Saraiva. 2018. Staphylococcus aureus haem biosynthesis and acquisition pathways are linked through haem monooxygenase IsdG. Mol. Microbiol. 109:385-400. doi: 10.1111/mmi.14060.

 

T.P.T. Dao, A. Brûlet, F. Fernandes, M. Er-Rafik, K. Ferji, R. Schweins, J.-P. Chapel, A. Fedorov, M. Schmutz, M. Prieto, O. Sandre, J.-F. Le Meins. 2017. Mixing Block Copolymers with Phospholipids at the Nanoscale: From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains, Langmuir 33: 1705–1715. doi:10.1021/acs.langmuir.6b04478 

 

T.P.T. Dao, F. Fernandes, E. Ibarboure, A. Vax, M. Prieto, and J. F. Le Meins. 2017. Membrane Properties Of Giant Polymer And Lipid Vesicles Obtained By Electroformation And PVA Gel-assisted Hydration Methods. Colloids Surf A Physicochem Eng Asp. 533: 347-353. doi:10.1016/j.colsurfa.2017.09.005

C. Pereira, B. Monteiro, J.P. Leal, M. Otis, M.H. Casimiro, L. Ferreira, F. Fernandes, C. Laia. 2017. A case of self-organization in highly emissive Eu(III) ionic liquids, Eur. J. Inorg. Chem. 28: 3429-3434. doi:10.1002/ejic.201700649

 

M.J. Sarmento, S.N.Pinto, A. Coutinho, M. Prieto, F. Fernandes. 2016. Accurate quantification of inter-domain partition coefficients in GUVs exhibiting lipid phase coexistence. RSC Adv. 6: 66641-66649. doi: 10.1039/C6RA13170K 

N. Bernardes, S. Abreu, F.A. Carvalho, F. Fernandes, N.C Santos, A.M. Fialho. 2016. Modulation of membrane properties of lung cancer cells by azurin enhances the sensitivity to EGFR-targeted therapy and decreased β1 integrin-mediated adhesion, Cell Cycle 15(11):1415-24. doi: 10.1080/15384101.2016.1172147.

D. Ferreira, D. Conceição, F. Fernandes, T. Sousa, R. Calhelha, I. Ferreira, P. Santos, L.F.V. Ferreira. 2016. Characterization of a squaraine/chitosan system for photodynamic therapy of cancer, J. Phys. Chem. B 120 (7): 1212–1220. doi:10.1021/acs.jpcb.5b11604.

T.P.T. Dao, F. Fernandes, R. Salva, M. Schmutz, M. Prieto, O. Sandre. 2015. Phase separation and nanodomain formation in hybrid polymer/lipid vesicles, ACS Macro Lett., 4: 182–186. doi:10.1021/mz500748f

F. Fernandes, A. Coutinho, M. Prieto, L.M.S. Loura. 2015. Electrostatically driven lipid-protein interaction: answers from FRET. Biochim. Biophys Acta - Biomembranes 1848: 1837–1848. doi: 10.1016/j.bbamem.2015.02.023.

A. Ferreira, I. Silva, F. Fernandes, F. R. Pilkington, M. Callaghan, S. McClean, and L. Moreira. 2015. The tyrosine kinase BceF and the phosphotyrosine phosphatase BceD of Burkholderia contaminans are required for efficient invasion and epithelial disruption of a cystic fibrosis lung epithelial cell line. Infect. Immun. 83:812-21. doi: 10.1128/IAI.02713-14.

 

M.J. Sarmento, A. Coutinho, A. Fedorov, M. Prieto, F. Fernandes. 2014. Ca2+ induces PI(4,5)P2 clusters on lipid bilayers at physiological PI(4,5)P2 and Ca2+ concentrations., Biochim. Biophys Acta - Biomembranes 1838, 3:822-830. doi: 10.1016/j.bbamem.2013.11.020

M.E. Monteiro, M.J. Sarmento, F. Fernandes. 2014. Role of calcium in membrane interactions by PI(4,5)P2-binding proteins., Biochem. Soc. Trans. 42 1441–1446. doi: 10.1042/BST20140149

A.M. Melo, L.M.S. Loura, F. Fernandes, J. Villalaín, M. Prieto, A. Coutinho. 2014 Electrostatically driven lipid–lysozyme mixed fibers display a multilamellar structure without amyloid features, Soft Matter 10, 840-850. doi: 10.1039/c3sm52586d.

S. Magalhães, S. Duarte, G.A. Monteiro, F. Fernandes. 2014. Quantitative evaluation of DNA dissociation from liposome carriers and DNA escape from endosomes during lipid-mediated gene delivery. Hum. Gene Ther. Methods 25: 303-313. doi: 10.1089/hgtb.2014.080

D. Mil-Homens, M.I. Leça, F. Fernandes, S. Pinto, A. Fialho. 2014. Characterization of BCAM0224, a multifunctional trimeric autotransporter from the human pathogen Burkholderia cenocepacia. J. Bacteriol. 196: 1968-79. doi: 10.1128/JB.00061-14.

T. Ribeiro, S. Raja, A.S. Rodrigues, F. Fernandes, C. Baleizão and J.P.S. Farinha. 2014. NIR and visible perylenediimide-silica nanoparticles for laser scanning bioimaging, Dyes Pigments. 110: 227-234. doi: 10.1016/j.dyepig.2014.03.026

 

J. Mello-Vieira, T. Sousa, A. Coutinho, A. Fedorov, S.D. Lucas, R. Moreira, R.E. Castro, C.M. Rodrigues, M. Prieto, F. Fernandes. 2013. Cytotoxic bile acids, but not cytoprotective species, inhibit the ordering effect of cholesterol in model membranes at physiologically active concentrations, Biochim. Biophys Acta – Biomembranes 1828, 9: 2152 - 2163. doi: 10.1016/j.bbamem.2013.05.021

S.N. Pinto, F. Fernandes, A. Fedorov, A.H. Futerman, L.C. Silva, M. Prieto. 2013. A combined fluorescence spectroscopy, confocal and 2-photon microscopy approach to re-evaluate the properties of sphingolipid domains, Biochim. Biophys Acta - Biomembranes 1828, 9: 2099 - 2110. doi: 10.1016/j.bbamem.2013.05.011.

T. Ribeiro, R.Sebastian, A.S. Rodrigues, F. Fernandes, J.P.S. Farinha, C. Baleizão. 2013. High performance NIR fluorescent silica nanoparticles for bioimaging, RSC Advances 3, 24: 9171 – 9171. doi: 10.1039/c3ra41199k.

A.S. Rodrigues, T. Ribeiro, F. Fernandes, J.P.S Farinha, C. Baleizão. 2013. Intrinsically fluorescent silica nanocontainers: A promising theranostic platform, Microsc. Microanal. 19:1216-21. doi: 10.1017/S1431927613001517.

A. Ferreira, I. Silva, V. Oliveira, J. Becker, M. Givskov, R. Ryan, F. Fernandes, and L. Moreira. 2013. Comparative transcriptomic analysis of the Burkholderia cepacia Tyrosine Kinase bceF mutant reveals a role in tolerance to stress, biofilm formation, and virulence, Appl. Environ. Microbiol. 79, 9: 3009 - 3020. doi: 10.1128/AEM.00222-13.

L.M.S. Loura, M. Prieto, F. Fernandes. Förster resonance energy transfer as a tool for quantification of protein-lipid selectivity. Methods Mol Biol.974 (2013)219-32. ISBN 978-1-62703-274-2.

M.J. Sarmento, M. Prieto, F. Fernandes.  2012. Reorganization of lipid domain distribution in giant unilamellar vesicles upon immobilization with different membrane tethers, Biochim. Biophys Acta - Biomembranes 1818: 2605-2615. doi: 10.1016/j.bbamem.2012.05.028

F. Fernandes, M. Prieto, L.M.S. Loura. 2012. Advanced FRET Methodologies: Protein–Lipid Selectivity Detection and Quantification. In "Biochemical Roles of Eukaryotic Cell Surface Macromolecules". ed. Perumana R. Sudhakaran Avadhesha Surolia, 171 - 185. ISBN: 978-1-4614-3380-4. New York, NY: Springer New York.

F. Fernandes, L. Loura, M. Prieto. 2013. Biophysical studies of V-ATPase inhibitors in model membranes, in “A Toxicological/Pharmacological Approach to Chemico-Biological Interactions at a Membrane Level’ (Editor: A. Jurado), R. Signpost ISBN: 978-81-308-0494-1

L.M.S. Loura, M. Prieto, F. Fernandes. 2010. Quantification of protein-lipid selectivity using FRET, Eur. Biophys J. 39(4): 565-578. doi: 10.1007/s00249-009-0532-z

F. Fernandes, L.M.S. Loura, M. Prieto. 2010. Membrane microheterogeneity: Fluorescence resonance energy transfer characterization of lateral membrane domains, Eur. Biophys. J. 39:589–607. doi: 10.1007/s00249-009-0547-5.

F. Fernandes, L.M.S. Loura, F.J. Chichón, J.L. Carrascosa, A. Fedorov, and M. Prieto. 2008. Role of helix 0 of the N-BAR domain in membrane curvature generation.  Biophys. J. 94: 3065-73. doi: 10.1529/biophysj.107.113118.

L.M.S. Loura, F. Fernandes, A.C. Fernandes, J.P. Prates Ramalho. 2008. Effects of fluorescent probe NBD-PC on the structure, dynamics and phase transition of DPPC. A molecular dynamics and differential scanning calorimetry study. Biochim. Biophys. Acta - Biomembranes 1778: 491-501. doi: 10.1016/j.bbamem.2007.10.022

F. Fernandes, P. Neves, P. Gameiro, L.M.S. Loura, and M. Prieto. 2007. Ciprofloxacin interactions with bacterial protein OmpF: Modeling of FRET from a multi-tryptophan protein trimer. Biochim. Biophys. Acta – Biomembranes 1768: 2822-30. doi: 10.1016/j.bbamem.2007.07.016.

F. Fernandes, L. Loura, A. Fedorov, N. Dixon, T. P. Kee, M. Prieto, and M. Hemminga. 2006. Binding assays of inhibitors towards selected V-ATPAase domains. Biochim. Biophys. Acta – Biomembranes 1758: 1777-1786. doi: 10.1016/j.bbamem.2006.07.006.

F. Fernandes, L. M. S. Loura, and M. Prieto. 2006. Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine. J. Lipid Res. 47: 1521-1525. doi: 10.1194/jlr.M600121-JLR200.

F. Fernandes, L. Loura, R. Koehorst, N. Dixon, T.P. Kee, M. Hemminga, and M. Prieto. 2006. Interaction of the indole class of V-ATPase inhibitors with lipid bilayers. 45: 5271-5279. doi: 10.1021/bi0522753.

F. Fernandes, L.M.S. Loura, R. Koehorst, R. Spruijt, M. Hemminga, A. Fedorov, and M. Prieto. 2004. Quantification of protein-lipid selectivity using FRET. Application to the M13 major coat protein. 87: 344-352. doi: 10.1529/biophysj.104.040337.

F. Fernandes, L.M.S. Loura, M. Prieto, R. Koehorst, R. Spruijt, and M. Hemminga. 2003. Dependence of M13 major coat protein oligomerization and lateral segregation on bilayer composition. Biophys. J. 85: 2430-2431.doi: 10.1016/S0006-3495(03)74666-9.